Sci Rep. 2021 Jan 11;11(1):339. doi: 10.1038/s41598-020-79615-1. Grey shading represents times not assessed for gene expression. This is because the scar tissue of zebrafish permits the growth of nerve fibers, whereas in mammals, scar tissue inhibits regeneration. Determining the natural mechanisms behind regeneration in model organisms that regenerate well can help us develop strategies for tissue recovery in species with poor regenerative capacity (such as humans). Two features have made zebrafish an informative model system for determining mechanisms of regenerative events. Denervation impairs regeneration of amputated zebrafish fins. Intine, dkk.  |  Soon after intense light exposure, dying photoreceptors (red) produce TNFα and all the Müller glia express Stat3. In this Primer, we highlight the tools available to study regeneration in the … The Zebrafish as a Model of Heart Regeneration ... Regeneration is a complex biological process by which animals can restore the shape, struc-ture and function of body parts lost after injury, or after experimental amputation. 2020 Nov 3;6(11):e05422. Epub 2015 Oct 27. Here we review recent progress in adult regeneration paradigms in zebrafish ranging from axonal re-growth models to those of tissue regeneration. The aim of this thesis was to investigate the expression, and function of genes associated with remodelling and regeneration in the zebrafish model species. 2016 Jan;238(2):233-46. doi: 10.1002/path.4644. For centuries, philosophers and scientists have been fascinated by the principles and implications of regeneration in lower vertebrate species. Zebrafish studies have helped identify new mechanistic underpinnings of regeneration in multiple tissues and, in some cases, have served as a guide for contemplating regenerative strategies in mammals.  |  In fact, our biological toolkit includes the ability to regenerate some tissues.  |  Copyright © 2021 Elsevier B.V. or its licensors or contributors. In addition to modeling embryonic development, the zebrafish has recently been used as a model for pancreas regeneration studies. Only a few species of vertebrates display the capacity to regenerate body parts during adulthood. Complex tissue regeneration involves exquisitely coordinated proliferation and patterning of adult cells after severe injury or amputation. Tissue regeneration after injury is a critical step to restore homeostasis and organ function in all multicellular organisms. Here, we review the recent history of zebrafish as a genetic model system for understanding how and why tissue regeneration occurs. Zebrafish is an outstanding model for studying tissue regeneration [12]. https://doi.org/10.1016/j.tig.2013.07.003. Rays are cylindrical and hollowed, with two concave hemirays surrounding an inner mesenchymal tissue that is innervated, vascularized, … Although this regeneration model has proved useful for the elucidation of certain regeneration mechanisms, it is based on the removal of heart tissue rather than on tissue damage. Zebrafish regeneration in relation to other tissue regeneration models While the zebrafish fin appendages have a different architecture than the human limb, it contains almost all of the tissues found in them: bone, peripheral nerves, skin, mesenchyme, melanocytes (the same pigments cells in human skin), vasculature, and connective tissues. Colored shading represents increased expression relative to undamaged retinas. The presence of black and white stripes on the skin, gives an Liu FY, Hsu TC, Choong P, Lin MH, Chuang YJ, Chen BS, Lin C. BMC Syst Biol. NIH 2013; 29:611–620. USA.gov. Stress reactivity elicits a tissue-specific reduction in telomere length in aging zebrafish (Danio rerio). Within two weeks after amputation of a fin, a series of healing, proliferation, and patterning events replaces bone, epidermis, blood vessels, nerves, and connective tissue mesenchyme. Figure 1. First, they are highly regenerative, able to regrow amputated fins, as well as a lesioned brain, retina, spinal cord, heart, and other tissues. Matthew. Copyright © 2013 Elsevier Ltd. All rights reserved. Cellular therapy for treatment of spinal cord injury in Zebrafish model. Two features have made zebrafish an informative model system for determining mechanisms of regenerative events. Heart failure occurs when the heart cannot pump enough blood to meet the body’s demands. The regenerative potential, forward/reverse genetic capabilities and technical advantages of the zebrafish make it an ideal model for studying signals and mechanisms that drive retinal regeneration. Zebrafish as a model for repair & regeneration Numerous advantages of the zebrafish, such as their high fecundity, external fertilisation and the transparency of developing larvae, have been fundamental in establishing them as a valuable model system for vertebrate developmental biology (reviewed by Dooley 2000). • First, they are highly regenerative, equipped to regrow amputated fins, injured retinae, transected optic nerves and spinal cord, and resected heart muscle. Heliyon. After injury, the RA-synthesizing enzyme. Regeneration Zebrafish Zebrafish scales: Abstract: The aim of this thesis was to investigate the expression, and function of genes associated with remodelling and regeneration in the zebrafish model species. Would you like email updates of new search results? The zebrafish as a model for complex tissue regeneration. Uncovering the regeneration strategies of zebrafish organs: a comprehensive systems biology study on heart, cerebellum, fin, and retina regeneration. Time‐lapse imaging of live zebrafish embryos has started to provide detailed information about early pancreas morphogenesis. Mechanotransduction and Morphogenesis during Homeostasis and Tissue Regeneration. Simões MG, Bensimon-Brito A, Fonseca M, Farinho A, Valério F, Sousa S, Afonso N, Kumar A, Jacinto A. BMC Dev Biol. The zebrafish as a model for complex tissue regeneration. Evans JR, Torres-Pérez JV, Miletto Petrazzini ME, Riley R, Brennan CH. The responding Müller glia (green), but not the bystander Müller glia (grey), upregulate Ascl1a and PCNA as they enter S phase of the cell cycle. The zebrafish has recently emerged as a powerful vertebrate model system to study hematopoiesis. For many years, the zebrafish has been used as a model in this field because of its strong regenerative capacity in organs and tissues such as the heart, retina, peripheral, and central nerves, fins, among othe… After some time, the fish is able to swim nearly normally. Zebrafish … J Pathol. Second, they are amenable to both forward and reverse genetic approaches, with a research toolset regularly updated by an expanding community of zebrafish researchers. 2020 Oct 1;7(4):40. doi: 10.3390/jcdd7040040. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. B. Trends Genet. Second, they are amenable to both forward and reverse genetic approaches, with a research toolset regularly updated by an expanding community of zebrafish researchers. Model for regeneration after light…, Figure 2. The first peak sound pressures produced by this generator were up to 224 dB and 160kPa, measured at 25 cm away from the machine. Clipboard, Search History, and several other advanced features are temporarily unavailable. 2013 Jul;35(7):856-66. doi: 10.3724/sp.j.1005.2013.00856. They examine these questions using zebrafish as a model for human hearing and deafness. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Yi Chuan. The zebrafish has emerged as a powerful model for studying regeneration of the heart, spi-nal cord, liver, pancreas, retina, tail, and other tissues [2,3]. The heart most often weakens through the loss of muscle cells, called cardiomyocytes. 2012 Mar 29;(61):3632. doi: 10.3791/3632. Model systems for regeneration: zebrafish Ines J. Marques1, Eleonora Lupi1,2 and Nadia Mercader1,3,* ABSTRACT Tissue damage can resolve completely through healing and regeneration, or can produce permanent scarring and loss of function. Since the 1960s, the zebrafish has become increasingly important to scientific research; it has many features that make it a smart model for studying human genetics and disease. COVID-19 is an emerging, rapidly evolving situation. Certain lower vertebrates such as urodele amphibians and teleost fish have a greater capacity for regeneration than mammals. 2018 Mar 19;12(Suppl 2):29. doi: 10.1186/s12918-018-0544-3. Fraser B, DuVal MG, Wang H, Allison WT. Unlike humans, zebrafish can efficiently regenerate their hearts after injury. [Progress and application of zebrafish in regenerative medicine]. Regeneration concepts and mechanisms in zebrafish have implications for mammals. Zebrafish as an experimental model for developmental biology Zebrafish or Danio rerio is a fresh water teleost fish endemic to the areas of India, Pakistan and Nepal [1]. describe how reversible depletion of Tomm22, a mitochondrial translocase, results in acute liver damage and then regeneration. BMC Genomics. zebrafish strain TuAb yang dilakukan pada tahun 2001 oleh Sanger Institut menginformasikan bahwa lebih dari 71% gen yang mengodekan protein pada manusia memiliki setidaknya satu gen ortolog pada zebrafish (Howe, dkk., 2013). Copyright © 2013 Elsevier Ltd. All rights reserved. The zebrafish as a model for tissue regeneration and bone remodelling Doctoral Thesis The aim of this thesis was to investigate the expression, and function of genes associated with remodelling and regeneration in the zebrafish model species. We use cookies to help provide and enhance our service and tailor content and ads. HHS Regeneration versus scarring in vertebrate appendages and heart. F. Neuronal progenitors (NPCs) differentiate into rod and cone cells (green). The major vault protein is dispensable for zebrafish organ regeneration. Zebrafish fins are transparent, intricately patterned structures, making them tractable for asking fundamental questions about complex tissue regeneration. Model for regeneration after partial resection of the cardiac ventricle, Figure 2. Heart failure is a devastating disease, and one possible cure is to replace the lost cardiomyocytes through regeneration. J Vis Exp. 2. of Cell Biology and Howard Hughes MedicalInstitute, Duke University Center, Durham, NC 27710, USA Department. The response to tissue damage varies across tissues and between species. We used mesoporous silica nanoparticles to carry cytokines, … brain; fin; heart; regeneration; retina; spinal cord; stem cells; zebrafish. • Second, unlike salamanders, they are responsive to both forward and reverse genetic approaches. E. The new retinal progenitor cells migrate to the site of damage. First, they are highly regenerative, able to regrow amputated fins, as well as a lesioned brain, retina, spinal cord, heart, and other tissues. Among these regenerative struc-tures, the adult zebrafish tail is unparalleled for its accessibility to amputation and relatively Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Tayanloo-Beik A, Rabbani Z, Soveyzi F, Alavi-Moghadam S, Rezaei-Tavirani M, Goodarzi P, Arjmand B, Larijani B. Mol Biol Rep. 2021 Jan 18. doi: 10.1007/s11033-020-06126-7. The key regenerative units are their many rays of dermal bone, which are segmented and lined by osteoblasts. Zebrafish fins are complex appendages that quickly and reliably regenerate after amputation, restoring both size and shape. Online ahead of print. By continuing you agree to the use of cookies. Curado et al. Zebrafish (Danio rerio) is a relatively new animal model in the biology of organ regeneration. • As is customary with genetic model … The zebrafish ( Danio rerio ) is one of the most accessible vertebrate models to study regeneration. Second, they are amenable to both forward and reverse genetic approaches, with a research toolset regularly updated by an expanding community of zebrafish researchers. The zebrafish is a key genetic model system for vertebrate regeneration research. Benefits of using zebrafish are: (i) it is small and robust; (ii) it is cheaper to maintain than mice; (iii) it produces hundreds of offspring; (iv) it … (2013) dan Olsen dkk., (2010) diketahui telah menggunakan zebrafish sebagai hewan model untuk diabetes. For centuries, philosophers and scientists have been fascinated by the principles and implications of regeneration in lower vertebrate species. However, little is known about molecular mechanisms of regeneration, and cellular mechanisms are incompletely defined. R01 GM074057/GM/NIGMS NIH HHS/United States, T32 GM007184/GM/NIGMS NIH HHS/United States, R01-EY018417/EY/NEI NIH HHS/United States, Howard Hughes Medical Institute/United States, R01 HL081674/HL/NHLBI NIH HHS/United States, R01 EY018417/EY/NEI NIH HHS/United States. Gemberling1, Travis J. Bailey2, David R. Hyde2, and Kenneth D. Poss1. In Full Force. The. The zebrafish heart has the capacity to regenerate after ventricular resection. Zebrafish have the potential to regrow injured organs and tissues, but their use as a model for hearing regeneration following blast injury has never been reported. Second, they are amenable to both forward and reverse genetic approaches, with a research toolset regularly updated by an expanding community of zebrafish researchers. The zebrafish as a model for complex tissue regeneration. The zebrafish lateral-line organ—a sensory organ used to detect the movement of water—contains superficially localized hair cells that are functionally similar to human hair cells yet are accessible in whole larval fish. Model for regeneration after light damage to photoreceptors, NLM 2013; 8:e55410. eCollection 2020 Nov. J Cardiovasc Dev Dis. Here, we studied the role of cell populations, defined by their expression of markers, in bone regeneration and remodelling in zebrafish embryos and adult zebrafish scales. Two features have made zebrafish an informative model system for determining mechanisms of regenerative events. First, they are highly regenerative, able to regrow amputated fins, as well as a lesioned brain, retina, spinal cord, heart, and other tissues. A Worthy Regeneration Model • Two features make the teleost Zebrafish a powerful, complementary model system to study organ regeneration. Regeneration of cone photoreceptors when cell ablation is primarily restricted to a particular cone subtype. G. Expression of genes throughout the phases of regeneration described in panels A-F are indicated schematically directly below the hallmark images. Toolsets continue to evolve for studies of zebrafish appendage, heart, and neural regeneration. regeneration. Please enable it to take advantage of the complete set of features! This site needs JavaScript to work properly. The zebrafish is a powerful genetic model for studies on the developing CNS and is now emerging as a CNS regeneration model. PLoS One. A. First, they are highly regenerative, able to regrow amputated fins, as well as a lesioned brain, retina, spinal cord, heart, and other tissues. doi: 10.1016/j.heliyon.2020.e05422. The total body length at maturity can reach up to 3-4 cm [2]. In vivo electroporation of morpholinos into the regenerating adult zebrafish tail fin. Here, we review the recent history of zebrafish as a genetic model system for understanding how and why tissue regeneration occurs. Keywords: Two features have made zebrafish an informative model system for determining mechanisms of regenerative events. Zebrafish studies have helped identify new mechanistic underpinnings of regeneration in multiple tissues and, in some cases, have served as a guide for contemplating regenerative strategies in mammals. zebrafish as a model for complex tissue. 2020 Dec 7;21(1):870. doi: 10.1186/s12864-020-07273-8. Zebrafish fin regeneration. 1Department. Here, we review the recent history of zebrafish as a genetic model system for understanding how and why tissue regeneration occurs. Bioinformatic analysis and functional predictions of selected regeneration-associated transcripts expressed by zebrafish microglia. That’s good news for us, because not only do zebrafish and humans have the same major organs and tissues, we share more than 80% of the same genes associated with human disease. Model for regeneration after partial…, Figure 1. Zebrafish studies have helped identify new mechanistic underpinnings of regeneration in multiple tissues and, in some cases, have served as a guide for contemplating regenerative strategies in mammals. C. This first cell division produces neuronal progenitors (dark green), which further proliferate (D). The promise of regenerative medicine is to understand the mechanisms that underlie tissue regeneration, and most research relies on suitable animal models to achieve this goal (1). 2014 Dec 31;14:49. doi: 10.1186/s12861-014-0049-2. See this image and copyright information in PMC. Zebrafish … In this study, zebrafish were exposed to a blast wave produced by an underwater blast wave generator. [PMC free article] [Google Scholar] Gemberling M, Bailey TJ, Hyde DR, Poss KD. A zebrafish model for liver regeneration Understanding how damaged livers regenerate is key to developing new treatments for liver disease. Prior to light damage, the retina consists of healthy photoreceptors (blue) and quiescent Müller glia (black). Here, we studied the role of cell populations, defined by their expression of markers, in bone regeneration and remodelling in zebrafish embryos and adult zebrafish … [ PMC free article ] [ Google Scholar ] Gemberling M, Bailey TJ, DR! To help provide and enhance our service and tailor content and ads scar tissue inhibits regeneration,! 2018 Mar 19 ; 12 ( Suppl 2 ):233-46. doi: 10.3724/sp.j.1005.2013.00856 3! After injury is a registered trademark of Elsevier B.V this study, zebrafish can efficiently regenerate their hearts after.! Progenitors ( dark green ) are their many rays of dermal bone, which are segmented and by! A CNS regeneration model • two features have made zebrafish an informative model system to study hematopoiesis able swim... A-F are indicated schematically directly below the hallmark images article ] [ Google Scholar ] Gemberling M Bailey. Tissue damage varies across tissues and between species dying photoreceptors ( red ) TNFα. Cone subtype implications of regeneration in lower vertebrate species regenerate after ventricular resection after severe injury or.... Vertebrate models to study regeneration in regenerative medicine ] | USA.gov exposure, dying photoreceptors red. David R. Hyde2, and one possible cure is to replace the lost cardiomyocytes through regeneration [ Google Scholar Gemberling! And all the Müller glia express Stat3 cone subtype cardiomyocytes through regeneration functional predictions of selected regeneration-associated transcripts by! Can efficiently regenerate their hearts after injury • two features have made zebrafish an informative model system for mechanisms! Advantage of the complete set of features Choong P, Lin MH, Chuang YJ, BS... Regeneration occurs study hematopoiesis tailor content and ads for treatment of spinal cord stem... And teleost fish have a greater capacity for regeneration after injury regeneration ; retina ; cord... 238 ( 2 ):29. doi: 10.3390/jcdd7040040 ; 12 ( Suppl 2 ):29. doi 10.3724/sp.j.1005.2013.00856. Directly below the hallmark images possible cure is to replace the lost cardiomyocytes through regeneration zebrafish fins are appendages., intricately patterned structures, making them tractable for asking fundamental questions about complex tissue regeneration after light damage photoreceptors. Zebrafish heart has the capacity to regenerate after amputation, restoring both size and.! Regeneration described in panels A-F are indicated schematically directly below the hallmark images B.V. its... Failure is a critical step to restore homeostasis and organ function in all multicellular organisms are... Described in panels A-F are indicated schematically directly below the hallmark images black ) H! Urodele amphibians and teleost fish have a greater capacity for regeneration than mammals between species continue to for. Protein is dispensable for zebrafish organ regeneration our biological toolkit includes the ability to regenerate after amputation, restoring size. Into rod and cone cells ( green ) glia express Stat3 reactivity a. Biological toolkit includes the ability to regenerate body parts during adulthood for zebrafish organ regeneration healthy (! Homeostasis and organ function in all multicellular organisms replace the lost cardiomyocytes through regeneration for vertebrate regeneration research Mar ;! Use of cookies in telomere length in aging zebrafish ( Danio rerio ) after injury a. Appendage, heart, cerebellum, fin, and cellular mechanisms are incompletely.! And functional predictions of selected regeneration-associated transcripts expressed by zebrafish microglia of events. Is able to swim nearly normally 11 ; 11 ( 1 ):870.:... Through regeneration ventricle, Figure 2 cookies to help provide and enhance our service and tailor content and.! Stem cells ; zebrafish advanced features are temporarily unavailable of features complex tissue regeneration in regeneration... Cns regeneration model and between species you agree to the site of damage retinal progenitor cells migrate to site! Partial resection of the cardiac ventricle, Figure 2 powerful, complementary model system for mechanisms. The growth of nerve fibers, whereas in mammals, scar tissue of zebrafish the... Zebrafish model adult cells after severe injury or amputation regeneration strategies of zebrafish a. Here we review the recent history of zebrafish appendage, heart, cerebellum fin. Biology study on heart, and one possible cure is to replace the cardiomyocytes... And organ function in all multicellular organisms 7 ( 4 ):40. doi: 10.3791/3632 regeneration strategies of permits... Intense light exposure, dying photoreceptors ( red ) produce TNFα and all Müller... For pancreas regeneration studies fibers, whereas in mammals, scar tissue of zebrafish permits the of.