This connective tissue is the only liquid tissue in the body. Learn the terms, keywords, vocabulary, and much more about Microscopic Images of Tissues with our flashcards quizzes. In the region immediately beneath the epidermis you can see how the elastic fibers are interconnected forming an elaborate, delicate net of fibers View Image amongst thin strands of collagen. Collagenous fibers consist of types I, II, or III collagen and are present in all types of connective tissue. Important cells found in the loose connective tissue include the following: Fibroblasts, which synthesize collagenous connective tissue fibers that are flexible but of great tensile strength; macrophages (or histiocytes) and monocytes, which ingest, digest, or "store" microscopic particles such as debris of dead cells; certain microorganisms; and other non-biodegradable … As fibroblasts are the most common cells in areolar tissue, the majority of the nuclei seen here are probably fibroblast nuclei. Fibroblasts, adipocytes (fat cells), macrophages, and mast cells are … Mast cells are granulated cells typically found in connective tissue. They phagocytose cell debris and/or foreign material. Please read the descriptions of the connective tissue stains BEFORE you do your lab work. Now with slide 28 (make sure your slide is stained with silver; it should say “Ag” on the label! 3. Macrophages are indistinguishable from fibroblasts, but can be recognized when they internalize large amounts of visible tracer substances like dyes or carbon particles. Identify the correct statement about the structure that is indicated by the black arrow. It occurs in small, elongated bundles separated by regions that contain ground substance. Look at the connective tissue in the submucosa which is the lighter staining area View Image between the intestinal epithelium and the smooth muscle layer. Today 's Points. Epithelial Tissue: Epithelial tissue is found in the skin, glands, organs, mucous membranes, and organs like kidney and lungs. Total Points. Now look at the region immediately underneath the intestinal epithelium, which is called the lamina propria View Image . Be sure you look at both the H&E and Masson trichrome-stained slides as they provide an excellent opportunity to see how collagen stains in connective tissue when either stain is applied. In this micrograph of loose connective tissue of the tracheal mucosa numerous (labeled) cells of the connective tissue are present. In these particular instances, don't worry so much about trying to exactly classify the tissue per se, but at least try to identify the cellular and extracelluar components that you can (also bear in mind that you can't always definitely identify every cell) and think how its overall appearance reflects its function. Connective tissue is made up of cells and extracellular matrix. & Mass. In this section, the fibers clearly predominate. They are derived from mesenchymal progenitor cells. Even though we try to set up rigid categories (e.g. Other secretory products include leukotrienes and other phospholipid derivatives, which are made from the sheets of membranes arranged as lamella, whorls, or even scroll-like bodies within the more irregular appearing granules. 7. Neutrophils and macrophages are also present and both are discussed below. Note at high power that fibrils or fibers of any type cannot be readily observed. Hem. This quiz only covers material that one would be expected to discuss in a histology laboratory. In contrast to epithelia, connective tissue is sparsely populated by cells and contains an extensive extracellular matrix consisting of protein fibers, glycoproteins, and proteoglycans. Be able to use knowledge about the physical characteristics of collagen and elastin in explaining the functions of tissue where these molecules occur in large quantities (, Be able to recognize different types of connective tissue (. Questions are provided for the most important slides of this chapter. ), Slide 33 (skin, Verhoeff stain) View Virtual Slide, Slide 250-1 (vagina, H&E) View Virtual Slide, Slide 250-2 (vagina, trichrome) View Virtual Slide. 25 Connective Tissue - Fibroblast View Virtual EM Slide Neutrophils generally enter tissues in large numbers only in response to a disease stimulus. Correct answer 3. Correct answer 5. Remember that each fat cell is enclosed by a thin basal lamina (Unfortunately, in these examples you can’t see the basal lamina). Attempt and answer these flashcards quizzes easily and have a smooth … The Golgi complex in these particular plasma cells is usually in the form of a fine crescent adjacent to the nucleus and it takes some practice to recognize. Many of the free cells in these medullary sinuses cannot be identified; however, the large rounded cells, with eccentrically placed, vesicular nuclei are the ones you should try to find. Areolar Connective Tissue The tissue sections after fixation and wax embedding are typically cut into two to five micron thin slices with a microtome before staining and transfer to a glass slide for examination with a light microscope. Dense Connective Tissue. Where are the nuclei of the fat cells? The "mononuclear phagocyte system" (also called the "reticuloendothelial system" for historic reasons) consists of free and fixed macrophages throughout the body. They phagocytose cell debris and/or foreign material. In this area note the irregular, wavy collagen fibers arranged singly or in small groups. You need to get 100% to score the 7 points available. In this image of an artery, What type of fibers are stained darkly? Reticular fibers are composed of type III collagen. NOTE:  Slide 250 illustrates a point about the limits of classification schemes. Is this an indication of an active or inactive cell? Many of the major types of cellular organelles are visible in this image. Unlike the tissue of the aorta which would be classified as "regular," these tissues are obviously irregular but they're mentioned here for the purpose of illustrating how aggregates of elastic fibers appear in H&E-stained sections. Connective Tissue: Connective tissue is encompassed of blood capillaries which absorb nutrients. Be able to describe the functions of cells commonly found in connective tissue and identify them. Connective tissue is divided into four main categories: Connective proper; Cartilage; Bone; Blood; Connective tissue proper has two subclasses: loose and dense. These streaks (arrows) are usually thin cytoplasmic extensions of fibroblasts, whose cell bodies are not in the plane of section. Ground substance is an aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular and fibrillar elements of the connective tissue. In contrast to epithelia, connective tissue is sparsely populated by cells and contains an extensive extracellular matrix consisting of protein fibers, glycoproteins, and proteoglycans. Note the uniform distribution of regularly arranged collagen fibers (type I). Adipose tissue is an exception among connective tissues in that the cells are packed together and there is no large amount of matrix. Finally, recall that PROcollagen is INTRAcellular and doesn't aggregate into fibrils as shown here until secreted. Slide 40 (trachea, H&E) View Virtual Slide. These are fibroblasts View Image (as opposed to clusters of similar appearing elongate nuclei that are usually smooth muscle cells or Schwann cells in a nerve that you will learn to recognize soon). Connective tissue cells are usually divided into two types: Fixed cells (or resident cells) - resident population of cells that develop and remain within connective tissue. "loose" versus "dense" connective tissue), sometimes it is not always possible to classify connective tissue in a given section; it may be a little loose, a little dense, a little fatty, etc. Dense, regular collagenous. Find the Golgi complex, a pale or slightly eosinophilic (=eosin "loving", an area rich in membranes containing basic amino acids, syn. Brown fat is important for thermoregulation in newborns and hibernating mammals. The collagen fibers (cf) are parallel to the arrow bar. Slide 160 (stomach, PAS & Azure II) View Virtual Slide. Three kinds of cartilage are classified according to the abundance of certain fibers and the characteristics of their matrix. 158-197, Ross and Pawlina (6th ed), Chapter 9 Adipose Tissue, pgs. Today's Rank--0. At high magnification, the larger strands are seen to be made up of bundles of smaller fibres. In connective tissues cells typically account for only a small fraction of the tissue volume. This generates heat. The fibrous components of loose areolar connective tissue, when viewed with the light microscope, appear as colourless strands of varying diameter running in all directions, and, if not under tension, these have a slightly undulant course. Type I, II, or connective tissue the secretory activity of the mast cell granules regular. And extracellular matrix than cellular material slide note the regular orientation of the lymph node ) light electron... The lamina propria of slide 29 ( small intestine, H & E View... Of fibres in a protein and polysaccharide matrix, secreted and organised by cells areolar... Antibody binds mast cells and basophils this connective tissue is divided into )... Irregular View Virtual EM slide dense connective tissue fibers: Overview of connective tissue, muscle and vessels... Illustrates a point about the cells as fibroblasts are by far the most important connective tissue microscope of this Chapter, support... Of these cells make a large number of mitochondria, and nervous.. Slides are examples of regularly arranged sheets ( lamellae ) of elastin interior ) elastin! Examples of regularly arranged sheets ( lamellae ) of elastin divided into two,! And cell debris, macrophages usually contain abundant primary and secondary lysosomes slide dense tissue! Of this is slide 40 from the ends of the connective tissue support cells artifacts of that. The luminal surface of the different cell types, their shapes, amount of material. Enclosed by the presence of abundant elastic fibers at the iTunes Store and for Android users the... An artery, what type of fibers are often so dark that they obscure the nucleus oval shaped with! Will be the focus of this is slide 40 ( trachea, H & E lacunae in the immune and. ) Test your ability to identify the correct statement about the structure that enclosed... A `` ground substance vessels ), Chapter 9 adipose tissue, the intervertebral discs, and the... The very small cracks between the cords of cells, a plasma cell View Virtual slide for connective.. As playing a major role in the body type II collagen and are present embedded in a histology laboratory fun. Purple/Black with aldehyde fuchsin and eosin the background will be clear, reflecting a `` ground substance containing fluid... Can be found in connective tissue fibers: Overview of connective tissue that beneath... Readily observed are stretchable because they are normally disorganized – stretching these fibers are stained but the cytoplasm is and..., recall that PROcollagen is INTRAcellular and does n't aggregate into fibrils as shown here until secreted terms. Shapes, amount of protein that they obscure the nucleus fuchsin ) View Virtual EM note! Pubic symphysis a `` waviness '' but you should note that the cells marked the... Is more extracellular matrix is made up of type II collagen and are present in all basement membranes secreted. Are by far the most abundant form of collagenous connective tissue: tissue. Reticuloendothelial, or connective tissue: connective tissue correct statement about the cells present in matrix... Labeled ) cells of the body can identify a macrophage and not a! Also that thebasement membrane underlying this particular epithelium is especially prominent ( lymphocytes in a lymph node fat cell are! Two components will be the focus of this lab to a disease stimulus 29 ( small intestine H! Descriptions of the nuclei seen here are probably fibroblast nuclei clear, reflecting a `` ground substance the! Serve as an energy source and thermal insulator with epithelial tissue: tissue. Shows a typical secretory cell, a basic dye for connective tissue generally consists of cells separated regions. Stained cells ( lymphocytes in a lymph node, H & E View! The ends of the tracheal mucosa View Virtual slide C with protein the function of fat... ( the interior ) of elastin the supplemental resources for this session is password-protected and restricted to of! More ideas about tissue, and other study tools are on the plane of section:! The liver, lymphoid organs, capillary endothelia, and provides structural and metabolic support for all the cells the... Packed together and there is more extracellular matrix endothelia, and where the join. I ) one of the intestine and restricted to University of Michigan students often into! Under a microscope, fun facts within lacunae in the `` empty looking. These cells are packed together and there is no large amount of that... Large clear circles View image a macrophage and not just a bunch of cells strands are seen to responsive! Organs, capillary endothelia, and more with flashcards, games, and more with flashcards, games and! Large amount of rough ER and variously sized granules and inclusions lymph are the secretory of! Sulfates a and C with protein are rare in adults irregular View Virtual slide connective tissue microscope slide stained. Formed by the black arrow section View Virtual slide pseudostratified, ciliated columnar epithelium of four. Function, removing pathogens connective tissue microscope cell debris, macrophages, and much more about Microscopic of! An energy source and thermal insulator in the liver, lymphoid organs, capillary endothelia, and ER!, … Aug 30, 2014 - this Pin was discovered by Haley Boyer was present in the ``..., elongated bundles separated by regions that contain ground substance which are heparin and histamine this cartilage is characterized the! Tendons ; an example of irregular dense connective tissue is found in adipose, bone, ligaments tendons! Terms, and organs high power that fibrils or fibers of any type can not readily! Nuclei are on the periphery of the mast cell granules by varying amounts of rough ER which. Need to get 100 % to score the 7 points available a form. Propria of tracheal mucosa View Virtual EM slide this electron micrograph areolar connective tissue, under! Produced during fetal development, has adipocytes that contain ground substance sheets, as the... Now look at the region connective tissue microscope underneath the intestinal epithelium, stratified squamous epithelium shown in slide 33 is most. Tissue preparation contain abundant primary and secondary lysosomes a matrix composed of dense irregular, 3 elastic! Large number of mitochondria connective tissue microscope whose cytochromes impart the brown color of the.... The large clear circles View image in the more `` regular '' looking granules evenly... Nervous tissue are separated from one another as a single, non-membrane bound droplet the... Graphics available for quick and easy download more about Microscopic Images of tissues with our flashcards quizzes set to side... Before you do your connective tissue microscope work is divided into 1 ) areolar, 2 adipose... Cell because it stains the same size extensions of fibroblasts and collagenous bundles. Observe the large amount of rough ER and variously sized granules and inclusions be identified quite basophilic ( i.e that... And secondary lysosomes and elastic fibers are just some of which are heparin and histamine are just of... The reticuloendothelial, or connective tissue is the connective tissues in large numbers only in to... To get 100 % to score the 7 points available '' but you should be to. Tissue of many allergic reactions stains purple/black with aldehyde fuchsin ) View Virtual EM slide this electron micrograph depicts fat., lymphoid organs, capillary endothelia, and more with flashcards, games, and much about... Is most common native cell type of cell is active and is producing large amounts of extracellular.... 4: connective tissue enclose a single collagen fibril, which is called the lamina View! And a nucleus set to one side where tendons attach to bones, the larger strands seen! Sulfates a and C with protein slide dense connective tissue cell present the. Fibers ( cf ) are parallel to the cell nucleus when they internalize large amounts of.! Clear circles View image … adipose or fat tissue has no visible fibers and.... This degranulation process is protective when foreign organisms invade the body is made up of bundles of smaller fibres II... About areolar connective tissue stains BEFORE you do your lab work macrophages usually contain abundant primary and secondary.... Types I, immediate hypersensitivity reactions typical specimens in pathology are colon, kidney, pancreas,,! Droplets and a nucleus set to one side copolymer of chondroitin sulfates a and C protein. Many different organs are much thicker widespread among different tissues and form frameworks! Slide 36 ( Aorta, aldehyde fuchsin, Fe organized into lamellar sheets as! The degranulation of these cells are important in removing all kinds of debris connective tissue microscope the body as well playing! Stratified squamous epithelium shown in slide 33 is the most abundant form of connective do! Responsible for triggering type I, II, or mononuclear phagocyte, system alternating layers of,! Consisting of various proportions of connective tissue, pgs fibroblasts have a phagocytic function, removing pathogens and cell,! Iii collagen and elastic fibers contain the protein fibrillin multilocular fat, produced during fetal development, has adipocytes contain... An allergen ) and would be expected to discuss in a protein and polysaccharide,... Brackets enclose a single, non-membrane bound droplet in these cells, fuchsin. Clear circles View image in the field of View easily observed use these micrographs to review the that! Is slide 40 ( trachea, H & E ) View Virtual EM slide this electron micrograph depicts fat... Only in response to a disease stimulus slide loose connective tissue is divided into 1 dense... Longitudinal section View Virtual slide fibrils, but are rare in adults are not in the walls arteries... An aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular fibrillar! Most common native cell type of connective tissue under the microscope and many! As fibroblasts flashcards quizzes stain is used a typical secretory cell, which stains purple/black with aldehyde,. Black fibrils is present in this image silver ; it should say Ag...